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KMID : 0923620120120010018
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Immune Network
2012 Volume.12 No. 1 p.18 ~ p.26
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The Analysis of Vitamin C Concentration in Organs of Gulo?/? Mice Upon Vitamin C Withdrawal
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Kim Hye-Min
Bae Se-Yeon
Yu Yeon-Sil
Kim Ye-Jin
Kim Hang-Rae
Hwang Young-Il
Kang Jae-Seung
Lee Wang-Jae
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Abstract
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Background: Vitamin C is an essential nutrient for maintaining human life. Vitamin C insufficiency in the plasma is closely related with the development of scurvy. However, in vivo kinetics of vitamin C regarding its storage and consumption is still largely unknown.
Methods: We used Gulo?/? mice, which cannot synthesize vitamin C like human. Vitamin C level in plasma and organs from Gulo?/? mice was examined, and it compared with the level of wild-type mice during 5 weeks.
Results: The significant weight loss of Gulo?/? mice was shown at 3 weeks after vitamin C withdrawal. However, there was no differences between wild-type and vitamin C-supplemented Gulo?/? mice (3.3 g/L in drinking water). The concentration of vitamin C in plasma and organs was significantly decreased at 1 week after vitamin C withdrawal. Vitamin C is preferentially deposited in adrenal gland, lymph node, lung, and brain. There were no significant changes in the numbers and CD4/CD8 ratio of splenocytes in Gulo?/? mice with vitamin C withdrawal for 4 weeks. And the architecture of spleen in Gulo?/? mice was disrupted at 5 weeks after vitamin C withdrawal.
Conclusion: The vitamin C level of Gulo?/? mice was considerably decreased from 1 week after vitamin C withdrawal. Vitamin C is preferentially stored in some organs such as brain, adrenal gland and lung.
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KEYWORD
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Vitamin C insufficiency, Gulo?/? mice, In vivo kinetics
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